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Establishing genomic tools and resources for Guizotia abyssinica (L.f.) Cass.—the development of a library of expressed sequence tags, microsatellite loci, and the sequencing of its chloroplast genome
Author(s) -
DEMPEWOLF HANNES,
KANE NOLAN C.,
OSTEVIK KATHERINE L.,
GELETA MULATU,
BARKER MICHAEL S.,
LAI ZHAO,
STEWART MEGAN L.,
BEKELE ENDASHAW,
ENGELS JOHANNES M. M.,
CRONK QUENTIN C. B.,
RIESEBERG LOREN H.
Publication year - 2010
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2010.02859.x
Subject(s) - biology , microsatellite , whole genome sequencing , genome , genetics , sequence (biology) , chloroplast , dna sequencing , computational biology , evolutionary biology , gene , allele
We present an EST library, chloroplast genome sequence, and nuclear microsatellite markers that were developed for the semi‐domesticated oilseed crop noug ( Guizotia abyssinica ) from Ethiopia. The EST library consists of 25 711 Sanger reads, assembled into 17 538 contigs and singletons, of which 4781 were functionally annotated using the Arabidopsis Information Resource (TAIR). The age distribution of duplicated genes in the EST library shows evidence of two paleopolyploidizations—a pattern that noug shares with several other species in the Heliantheae tribe (Compositae family). From the EST library, we selected 43 microsatellites and then designed and tested primers for their amplification. The number of microsatellite alleles varied between 2 and 10 (average 4.67), and the average observed and expected heterozygosities were 0.49 and 0.54, respectively. The chloroplast genome was sequenced de novo using Illumina’s sequencing technology and completed with traditional Sanger sequencing. No large re‐arrangements were found between the noug and sunflower chloroplast genomes, but 1.4% of sites have indels and 1.8% show sequence divergence between the two species. We identified 34 tRNAs, 4 rRNA sequences, and 80 coding sequences, including one region (trnH‐psbA) with 15% sequence divergence between noug and sunflower that may be particularly useful for phylogeographic studies in noug and its wild relatives.