Premium
New methods for species and sex determination in three sympatric Mustelids, Mustela itatsi, Mustela sibirica and Martes melampus
Author(s) -
SEKIGUCHI T.,
SASAKI H.,
KURIHARA Y.,
WATANABE S.,
MORIYAMA D.,
KUROSE N.,
MATSUKI R.,
YAMAZAKI K.,
SAEKI M.
Publication year - 2010
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2010.02842.x
Subject(s) - biology , mustelidae , sympatric speciation , weasel , zoology , primer (cosmetics) , ecology , chemistry , organic chemistry , predation
We developed novel species and sex determination methods for three Japanese mustelid species. We used DDX3Y to determine sex and generated a primer set to amplify both DDX3X and DDX3Y DNA in Mustela itatsi , M. sibirica and Martes melampus . To determine species and sex simultaneously, we generated fluorescence‐labelled primers that give different fragment lengths at D‐loop, DDX3X and DDX3Y of these three species using a DNA sequencer.