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Multiplex PCR and RFLP approaches for identification of rabbitfish ( Siganus ) species using mitochondrial gene regions
Author(s) -
RAVAGOGOTANCO R. G.,
MANGLICMOT M. T.,
PANTE M. J. R.
Publication year - 2010
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02829.x
Subject(s) - biology , amplicon , multiplex , multiplex polymerase chain reaction , mitochondrial dna , restriction fragment length polymorphism , cytochrome b , genetics , microbiology and biotechnology , gene , computational biology , zoology , polymerase chain reaction
Molecular assays are described for the identification of six rabbitfish ( Siganus ) species. A multiplex PCR assay using primers targeting the mitochondrial cytochrome b region simultaneously identifies four species: Siganus canaliculatus, S. fuscescens , S. javus, and S. spinus. Subsequent RFLP assays of multiplex amplicons differentiate between S. virgatus and S. corallinus based on diagnostic fragments from the mitochondrial cytochrome oxidase I region. Assays were validated with known specimens demonstrating accuracy of the molecular identification. Applied to morphologically indistinguishable early developmental stages, these assays can facilitate studies on species‐specific spatio‐temporal patterns of larval dispersal and population connectivity to aid fishery management.