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New chloroplast primers for intraspecific variation in Dicranum scoparium Hedw. (Dicranaceae) and amplification success in other bryophyte species
Author(s) -
LANG A.,
NACIRI Y.
Publication year - 2010
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02821.x
Subject(s) - biology , intraspecific competition , bryophyte , fern , botany , population , genetic variability , genetic variation , chloroplast dna , genetics , zoology , chloroplast , genotype , gene , demography , sociology
Primers for four loci that amplify cpDNA regions have been designed for population genetic analyses in Dicranum scoparium Hedw. and compared with trn L(UAA)5′exon‐ trn F. All loci showed intraspecific variation with a number of haplotypes ranging between two and six. trn H‐ psb A Dic showed an intercontinental disjunction, but no variability within the four Swiss populations surveyed, whereas the three remaining loci displayed intrapopulation variability in at least one population ( rps 19‐ rpl 2, rpo B, trn T‐ rps 4). These primers were additionally tested on 22 bryophytes and three fern species. The primers amplified mostly in mosses and liverworts, but less well in ferns, pointing to their evolutionary distance from the bryophytes.