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Multiplex 16S rRNA haplotype‐specific PCR, a rapid and convenient method for fish species identification: an application to West African Clupeiform larvae
Author(s) -
DURAND J. D.,
DIATTA M. A.,
DIOP K.,
TRAPE S.
Publication year - 2010
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02776.x
Subject(s) - biology , sardinella , engraulis , multiplex polymerase chain reaction , agarose gel electrophoresis , 16s ribosomal rna , sardine , zoology , polymerase chain reaction , fishery , fish <actinopterygii> , genetics , gene , anchovy
A multiplex haplotype‐specific polymerase chain reaction (MHS‐PCR) method was developed, which identified seven Clupeiform species living in the tropical Eastern Atlantic region: Sardinella aurita , Sardinella maderensis , Ethmalosa fimbriata , Sardina pilchardus , Engraulis encrasicholus , Pellonula leonensis and Ilisha africana . 16S rRNA fragments were amplified using a species‐specific set of primers, yielding species‐specific size fragments, and then separated using agarose gel electrophoresis, enabling direct visual identification of targeted species. This method provides an accurate, easy and rapid tool for identifying species within large Clupeiform samples. It is suitable for investigations on early Clupeiform stages, species and identification in fishery management in the tropical Eastern Atlantic area.