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Species‐specific RFLP pattern in the Heat Shock Protein26 gene (H sp 26): a single‐locus tool for species identification and experimental testing of habitat‐induced isolation in the New World Artemia species
Author(s) -
BERISTAIN P.,
GAJARDO G.,
BOSSIER P.
Publication year - 2010
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02744.x
Subject(s) - biology , anostraca , brine shrimp , restriction fragment length polymorphism , branchiopoda , locus (genetics) , gene , heat shock protein , habitat , genetics , zoology , ecology , crustacean , genotype , cladocera
The brine shrimp Artemia (Crustacea, Branchiopoda), a paradigmatic inhabitant of hypersaline lakes, has molecular features to survive under stressful conditions, such as the p26 heat shock protein. We report the RFLP fingerprinting pattern (four restriction enzymes) of a 217 bp fragment of exon2 of the H sp 26 gene in six Artemia franciscana and four Artemia persimilis populations , the most genetically divergent Artemia species co‐occurring in latitudinal extremes of Chile. The species‐specific RFLP pattern observed is a simple and cost‐effective single‐locus tool for species delimitation and experimental testing the habitat‐induced isolation barrier between them.