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Three nuclear genes for phylogenetic, SNP and population genetic studies of molluscs and other invertebrates
Author(s) -
AUDZIJONYTE A.,
VRIJENHOEK R. C.
Publication year - 2010
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02737.x
Subject(s) - biology , phylogenetic tree , genetics , nuclear gene , gene , intron , population , invertebrate , mitochondrial dna , ecology , demography , sociology
The study reports new primers capable of amplifying fragments from three nuclear protein‐coding genes in a variety of deep‐sea molluscs and annelids – adenine nucleotide translocase ( A nt ), calmodulin ( C al ) and cyclophilin A ( C yc A ). The A nt primers appear to be restricted to bivalve molluscs, whereas the C al and C yc A primers also amplified appropriate gene fragments from Lepetodrilus gastropod molluscs and Osedax polychaete worms. The amplified fragment of C al contains an intron in the molluscs, but no intron was detected in the A nt and C yc A fragments from any of the tested animals. DNA sequences generated by the three primer sets exhibited one to 15 single nucleotide polymorphism sites in deep‐sea vesicomyid clams and Osedax boneworms. The observed levels of polymorphism indicate that the genes are likely to be useful in both population genetic and phylogenetic analyses of different invertebrate taxa.