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Development of a molecular diagnostic system to discriminate Dreissena polymorpha (zebra mussel) and Dreissena bugensis (quagga mussel)
Author(s) -
HOY MARSHAL S.,
KELLY KEVIN,
RODRIGUEZ RUSTY J.
Publication year - 2010
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02727.x
Subject(s) - dreissena , biology , zebra mussel , primer (cosmetics) , zoology , mussel , ecology , mollusca , bivalvia , chemistry , organic chemistry
A 3‐primer PCR system was developed to discriminate invasive zebra ( Dreissena polymorpha ) and quagga ( Dreissena bugensis ) mussel. The system is based on: 1) universal primers that amplifies a region of the nuclear 28s rDNA gene from both species and 2) a species‐specific primer complementary to either zebra or quagga mussel. The species‐specific primers bind to sequences between the binding sites for the universal primers resulting in the amplification of two products from the target species and one product from the nontarget species. Therefore, nontarget products are positive amplification controls. The 3‐primer system accurately discriminated zebra and quagga mussels from seven geographically distinct populations.