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High‐resolution melting analysis (HRMA): a highly sensitive inexpensive genotyping alternative for population studies
Author(s) -
SMITH B. L.,
LU C.P.,
ALVARADO BREMER J. R.
Publication year - 2010
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02726.x
Subject(s) - biology , genotyping , high resolution melt , amplicon , genotype , population , genetics , microbiology and biotechnology , polymerase chain reaction , gene , demography , sociology
High‐resolution melting analysis (HRMA) is a highly sensitive closed‐tube genotyping method used primarily in clinical studies. As the method is rapid, inexpensive and amenable to high throughput, we decided to investigate its applicability to population studies. Small amplicons and unlabelled probes were used to genotype the nuclear genes, lactate dehydrogenase‐A ( ldh ‐A), myosin light chain‐2 ( mlc‐2 ), acidic ribosomal phosphoprotein P0 ( ARP ) and calmodulin ( CaM ) in populations of swordfish, Xiphias gladius . Results indicate that HRMA is a powerful genotyping tool to study wild populations.