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Identification of single copy nuclear DNA markers for North American pit vipers
Author(s) -
GIBBS H. LISLE,
DIAZ JOSE
Publication year - 2010
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02707.x
Subject(s) - biology , subspecies , crotalus , phylogenetic tree , evolutionary biology , viper , nuclear dna , genetics , genomic dna , zoology , mitochondrial dna , dna , gene , ecology , venom
We describe 18 single copy nuclear DNA loci (10 loci cloned from a Sistrurus catenatus catenatus genomic library and eight intron‐based loci amplified using conserved primers) that detect sequence variation in species from all genera ( Sistrurus , Agkistrodon and Crotalus ) of North American pit viper snakes. These loci (mean size in bp ± SE: 433 ± 51) show large numbers of phylogenetically informative sites across species (mean + SE: 10.2 ± 1.5), but limited variation within subspecies suggesting that they will be most useful for multilocus species‐level phylogenetic analyses in these snakes.