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Microsatellite primers for the four Galápagos mockingbird species ( Mimus parvulus , Mimus macdonaldi , Mimus melanotis and Mimus trifasciatus )
Author(s) -
HOECK P. E. A.,
BUCHER T. B.,
WANDELER P.,
KELLER L. F.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02704.x
Subject(s) - biology , microsatellite , inbreeding , loss of heterozygosity , locus (genetics) , zoology , allele , genetics , gene , population , demography , sociology
Nineteen di‐ and tetranucleotide and one trinucleotide microsatellite DNA markers were isolated from the Galápagos mockingbird ( Mimus parvulus ) and tested for cross‐species amplification in the other three mockingbird species in the Galápagos. In addition, primers for two microsatellite loci previously developed for Mimus polyglottos were redesigned to obtain shorter amplification fragments. The number of alleles per locus and species ranged from 1 to 8, and expected heterozygosity varied from 0.0 to 0.809. These microsatellite markers will be useful to study levels of inbreeding in different island populations.