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Isolation, characterization and chromosome locations of polymorphic black‐billed magpie Pica pica (Corvidae, AVES) microsatellite loci
Author(s) -
MARTÍNGÁLVEZ DAVID,
DAWSON DEBORAH A.,
HORSBURGH GAVIN J.,
BURKE TERRY
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02703.x
Subject(s) - biology , corvidae , microsatellite , passerine , zebra finch , genetics , population , evolutionary biology , zoology , gene , allele , demography , neuroscience , sociology
One‐hundred and two unique loci were isolated from a microsatellite‐enriched black‐billed magpie Pica pica genomic library. Sixteen of these new loci, along with 148 existing microsatellite passerine loci, were tested for polymorphism in four unrelated black‐billed magpies. We identified a total of 43 unique polymorphic loci (10 and 33 respectively) that we characterized in 24 unrelated individuals from a population in Guadix, Spain. The putative chromosomal locations of loci polymorphic in black‐billed magpie were assigned based on the location of their orthologues in the assembled zebra finch and chicken genomes.