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Microsatellite markers for Spergularia media (L.) C. Presl. (Caryophyllaceae) and their cross‐species transferability
Author(s) -
PRINZ KATHLEEN,
SCHIE STEPHAN,
DEBENER THOMAS,
HENSEN ISABELL,
WEISING KURT
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02680.x
Subject(s) - biology , caryophyllaceae , microsatellite , inbreeding , locus (genetics) , botany , perennial plant , halophyte , population , allele , genetics , ecology , salinity , demography , sociology , gene
Abstract We developed 11 polymorphic microsatellite markers for Spergularia media (Caryophyllaceae), a perennial halophyte of coastal salt meadows and continental areas of western Eurasia. The number of alleles per locus observed in a single population of 20 individuals from the German North Sea coast ranged from 3 to 20. Observed and expected heterozygosities ranged from 0.200 to 0.850 and from 0.278 to 0.936 respectively. Observed heterozygosities were lower than expected heterozygosities at all loci, presumably as a consequence of inbreeding. All markers cross‐amplified in the closely related S. salina . Of these, nine were polymorphic in the heterologous species. Only two primer pairs generated PCR products in S. diandra .