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Isolation and characterization of 12 novel DNA microsatellites in the western rainbowfish, Melanotaenia australis
Author(s) -
YOUNG MICHAEL J.,
SIMMONS LEIGH W.,
EVANS JONATHAN P.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02663.x
Subject(s) - biology , microsatellite , zoology , genetics , allele , evolutionary biology , inheritance (genetic algorithm) , gene
Microsatellites were isolated from the western rainbowfish, Melanotaenia australis , for use as molecular markers. Twelve polymorphic loci were found. When characterized using 32 individuals, these had between two and 24 alleles each, and observed heterozygosities ranged from 0.00 to 0.97. Ten loci were in Hardy–Weinberg equilibrium, and all of them showed independent inheritance. These loci will be useful for the study of molecular subdivision in this species.