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Isolation and characterization of the first eight microsatellite loci in Gammarus fossarum (Crustacea, Amphipoda) and cross‐amplification in Gammarus pulex and Gammarus orinos
Author(s) -
DANANCHER DELPHINE,
CELLOT BERNARD,
DOLÉDEC SYLVAIN,
REYNAUD DOMINIQUE
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02662.x
Subject(s) - gammarus pulex , biology , gammarus , amphipoda , pulex , microsatellite , zoology , crustacean , loss of heterozygosity , ecology , genetics , allele , gene , daphnia
We characterized the first microsatellite markers for Gammarus fossarum . Eight loci gave satisfactory amplification patterns in two stream populations (Southern France) with number of alleles ranging from 2 to 10 and expected heterozygosity from 0.076 to 0.857. We performed cross‐amplification in two closely related gammarid species, Gammarus pulex and Gammarus orinos . Among the eight tested microsatellite loci, four correctly amplified in G. pulex and three in G. orinos .