Premium
Development and characterization of 30 polymorphic microsatellite markers for the Atlantic surfclam, Spisula solidissima (Dillwyn, 1817)
Author(s) -
WANG YAN,
WANG AIMIN,
GUO XIMING
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02660.x
Subject(s) - biology , microsatellite , locus (genetics) , allele , genetics , population , null allele , bonferroni correction , population genetics , evolutionary biology , gene , statistics , demography , mathematics , sociology
Thirty polymorphic microsatellite markers were developed for the Atlantic surfclam, Spisula solidissima , from an enriched library and characterized in 24 clams from a wild population. The number of alleles ranged from 3 to 16 per locus. The expected and observed heterozygosities ranged from 0.1942 to 0.9238 and 0.0833 to 0.875 respectively. Six loci showed significant ( P < 0.05 after Bonferroni correction) deviation from Hardy–Weinberg equilibrium, probably because of the presence of null alleles. Three primer pairs amplified duplicated loci with two involving tandem mini‐satellite repeats. Most of the microsatellite markers developed here should be useful for genetic studies in this species.