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Isolation and characterization of polymorphic microsatellites in the Potato Tuber Moth Tecia solanivora (Povolny, 1973) (Lepidoptera: Gelechiidae)
Author(s) -
TORRESLEGUIZAMON M.,
SOLIGNAC M.,
VAUTRIN D.,
CAPDEVIELLEDULAC C.,
DUPAS S.,
SILVAIN J.F.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02656.x
Subject(s) - lepidoptera genitalia , biology , gelechiidae , microsatellite , allele , genetics , botany , evolutionary biology , gene
Nine polymorphic microsatellite markers were isolated from Tecia solanivora , one of the most serious pests of potato tubers in Central and South America. As found in other studies of Lepidoptera, development of microsatellites is a difficult task: in our case, despite the large number of clones sequenced (796), of which 70 were unique, only nine loci were found to be both variable, and in Hardy–Weinberg equilibrium, No null alleles were detected. The loci were tested in three other co‐occurring Gelechiidae species, one of which was variable. These loci will be used to provide a greater understanding of the genetic changes occurring during the invasive process in this species.