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Microsatellite markers for mungbean developed from sequence database
Author(s) -
SEEHALAK WORAPA,
SOMTA PRAKIT,
SOMMANAS WARUNEE,
SRINIVES PEERASAK
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02655.x
Subject(s) - biology , microsatellite , sequence (biology) , sequence database , computational biology , genetics , database , gene , allele , computer science
A novel set of microsatellite markers for mungbean [ Vigna radiata (L.) Wilczek] was developed from the public sequence database. Seventy‐eight primers were designed and evaluated for polymorphism among 22 cultivated accessions. Eight polymorphic loci detected two to three alleles per locus with an average of 2.25. The observed heterozygosity varied from 0.00 to 0.18, while the expected heterozygosity ranged from 0.09 to 0.46. Among them, all eight loci showed significant departuring from Hardy–Weinberg equilibrium, while four pairs of loci displayed significant pairwise linkage disequilibrium values. All eight loci except DMB‐SSR1 showed heterozygote deficiency.