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DNA barcoding of marine crustaceans from the Estuary and Gulf of St Lawrence: a regional‐scale approach
Author(s) -
RADULOVICI ADRIANA E.,
SAINTEMARIE BERNARD,
DUFRESNE FRANCE
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02643.x
Subject(s) - dna barcoding , biology , crustacean , estuary , species complex , intraspecific competition , genetic divergence , ecology , barcode , amphipoda , taxonomy (biology) , zoology , genetic diversity , phylogenetic tree , gene , population , demography , sociology , computer science , operating system , biochemistry
Marine crustaceans are known as a group with a high level of morphological and ecological diversity but are difficult to identify by traditional approaches and usually require the help of highly trained taxonomists. A faster identification method, DNA barcoding, was found to be an effective tool for species identification in many metazoan groups including some crustaceans. Here we expand the DNA barcode database with a case study involving 80 malacostracan species from the Estuary and Gulf of St Lawrence. DNA sequences for 460 specimens grouped into clusters corresponding to known morphological species in 95% of cases. Genetic distances between species were on average 25 times higher than within species. Intraspecific divergence was high (3.78–13.6%) in specimens belonging to four morphological species, suggesting the occurrence of cryptic species. Moreover, we detected the presence of an invasive amphipod species in the St Lawrence Estuary. This study reconfirms the usefulness of DNA barcoding for the identification of marine crustaceans.