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Isolation of 21 polymorphic microsatellite markers for the Virginia opossum ( Didelphis virginiana )
Author(s) -
FIKE JENNIFER A.,
BEASLEY JAMES C.,
RHODES, JR OLIN E.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02606.x
Subject(s) - didelphis , biology , opossum , microsatellite , isolation (microbiology) , zoology , genetics , ecology , microbiology and biotechnology , gene , allele
Twenty‐one polymorphic microsatellite markers were developed for the Virginia opossum ( Didelphis virginiana ). The number of alleles ranged from two to 13 and observed heterozygosities ranged from 0.464 to 0.964. Significant heterozygote deficiencies were observed at three loci and null alleles were detected at five loci. Evidence for gametic disequilibrium was observed between three sets of paired loci after a sequential Bonferroni correction was applied. These markers will enable us to investigate the mating tactics, movement behaviour and social structure of Virginia opossum populations inhabiting fragmented agricultural landscapes.