Premium
Characterization of dinucleotide microsatellite loci and confirmation of sexing primers for the bush dog ( Speothos venaticus )
Author(s) -
DEMATTEO KAREN E.,
PARKER PATRICIA G.,
EGGERT LORI S.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02603.x
Subject(s) - biology , sexing , microsatellite , loss of heterozygosity , locus (genetics) , linkage disequilibrium , genetics , allele , genetic diversity , genotype , population , zoology , evolutionary biology , haplotype , gene , demography , sociology
Bush dogs ( Speothos venaticus ) are small, threatened canids from Central and South America. The ability to conduct population‐level studies using noninvasive genetic samples would provide important information on this poorly understood species. We characterized eight dinucleotide microsatellite loci using samples from 15 captive bush dogs. Allelic diversity ranged from 2 to 5 alleles per locus, and observed heterozygosity ranged from 0.267 to 0.933. All loci were in Hardy–Weinberg equilibrium and no evidence of genotypic linkage disequilibrium was found. We determined that DBX6 and DBY7 , two canid‐specific molecular sexing primers, accurately indicate the sex of individual bush dog samples.