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Characterization of nine novel microsatellite markers from Brandt's vole ( Lasiopodomys brandtii )
Author(s) -
YUE LINGFEN,
WANG DAWEI,
HUANG BAOHUAN,
LIU XIAOHUI
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02601.x
Subject(s) - microsatellite , biology , loss of heterozygosity , locus (genetics) , genetics , allele , vole , gene , population , demography , sociology
Highly polymorphic microsatellite markers are the most powerful tools to infer kinship relations. In this study, a library enriched for (AC) n (AG) n and (AGAT) n was constructed for screening microsatellite markers in Brandt's vole ( Lasiopodomys brandtii ), and nine novel polymorphic microsatellite markers were developed and characterized. The number of alleles ranged from 4 to 11 per locus and the mean polymorphism information content was 0.7535. The observed and the expected heterozygosity values averaged 0.760 (0.554–0.908) and 0.7914 (0.718–0.845), respectively. Average nonexclusion probability for one candidate parent varied from 0.485 to 0.716. These nine novel markers are highly polymorphic and powerful enough for our future kinship analysis.