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New polymorphic microsatellite markers and development of mitotyping primers for West Mediterranean green toad species ( Bufo viridis subgroup)
Author(s) -
COLLIARD CAROLINE,
SICILIA ALESSANDRA,
MORITZ CRAIG,
PERRIN NICOLAS,
STÖCK MATTHIAS
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02600.x
Subject(s) - biology , microsatellite , zoology , bufo , toad , allele , genetics , ecology , gene
We report new polymorphic microsatellites for three species of Palearctic green toads ( Bufo viridis subgroup): 10 in B. balearicus and seven each in B. siculus and B. boulengeri . Diversity at these loci, measured for 27 B. balearicus , 23 B. siculus and 11 B. boulengeri , ranged from low to high (two to 10 alleles). Mitotyping primers, specific to the control region, which allow fast screening of parapatric Sicilian endemic B . siculus and Italian mainland‐origin B. balearicus, were developed.