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Isolation and characterization of 17 microsatellite loci for the house finch ( Carpodacus mexicanus )
Author(s) -
OH KEVIN P.,
BADYAEV ALEXANDER V.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02555.x
Subject(s) - biology , microsatellite , locus (genetics) , melanism , genetics , genotyping , hardy–weinberg principle , linkage disequilibrium , loss of heterozygosity , population genetics , passerine , allele , population , evolutionary biology , allele frequency , genotype , zoology , haplotype , gene , demography , sociology
Abstract The house finch ( Carpodacus mexicanus ) has emerged recently as a model species in studies of sexual selection, reproductive physiology, population genetics, and epizootic disease ecology. Here we describe 17 highly polymorphic microsatellite loci for this species. In a sample of 36 individuals, we observed an average of 16 alleles per locus and heterozygosity ranged from 0.61 to 0.97. One locus showed significant deviation from Hardy–Weinberg proportions, but no significant gametic disequilibrium was observed among any of the loci. Amplification by polymerase chain reaction was optimized under similar parameters across loci, thereby facilitating multiplexing and rapid multilocus genotyping.