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Isolation and characterization of 17 microsatellite loci for the Chinese longsnout catfish ( Leiocassis longirostris )
Author(s) -
XIAO MINGSONG,
YANG GUANG
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02554.x
Subject(s) - biology , microsatellite , linkage disequilibrium , genetics , locus (genetics) , catfish , genetic diversity , population , evolutionary biology , allele , haplotype , gene , fishery , fish <actinopterygii> , demography , sociology
We describe the isolation and development of 17 polymorphic dinucleotide microsatellite loci for the Chinese longsnout catfish ( Leiocassis longirostris ). All loci were polymorphic in the 30 individuals tested. The number of alleles per variable locus ranged from 6 to 18, with a mean of 11.71. Observed and expected heterozygosities ranged from 0.467 to 1.000 and from 0.540 to 0.929, respectively. Eight loci were found to have deviated from HWE in the sampled population after Bonferroni correction. Linkage disequilibrium tests revealed significant linkage between two loci (LLW5 and LL27). These microsatellite loci will be useful for revealing population structure, genetic diversity, and phylogeography of the Chinese longsnout catfish.