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Isolation and characterization of 10 microsatellite loci in the common dormouse Muscardinus avellanarius
Author(s) -
NAIM DARLINA MD.,
KEMP STEPHEN J.,
TELFER SANDRA,
WATTS PHILLIP C.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02551.x
Subject(s) - biology , microsatellite , loss of heterozygosity , genetic diversity , genetics , allele , linkage disequilibrium , evolutionary biology , population , haplotype , gene , demography , sociology
To assess the level and spatial pattern of genetic diversity of the common dormouse, Muscardinus avellanarius , we developed polymorphic microsatellite loci from partial genomic libraries enriched for microsatellite motifs. Ten dinucleotide polymorphic microsatellites were isolated and levels of genetic diversity were assessed in 139 individuals from Bontuchel, (Denbighshire, Wales). We observed high levels of heterozygosity (mean H O = 0.792 ± 0.077; mean H E = 0.730 ± 0.084) and a large number of alleles ( N a = 6–17). There was no evidence of deviations from Hardy–Weinberg conditions, or genotypic disequilibrium between any pairs of loci. This is the first description of microsatellite primers from a common dormouse and these loci are currently being used to quantify dormouse spatial genetic structure.