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Isolation and characterization of microsatellite loci in Symbiodinium B1/B184, the dinoflagellate symbiont of the Caribbean sea fan coral, Gorgonia ventalina
Author(s) -
ANDRAS JASON P.,
KIRK NATHAN L.,
COFFROTH MARY ALICE,
HARVELL C. DREW
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02549.x
Subject(s) - symbiodinium , biology , dinoflagellate , microsatellite , coral , genetic diversity , genus , evolutionary biology , population , zoology , ecology , genetics , symbiosis , gene , allele , bacteria , demography , sociology
Here we report primers targeting 10 microsatellite loci of dinoflagellates in the genus Symbiodinium (clade B1/B184) symbiotic with the Caribbean sea fan coral, Gorgonia ventalina . Primers were tested on 12 Symbiodinium B1/B184 cultures, as well as 40 genomic DNA extracts of G. ventalina tissue samples. All loci were polymorphic with allelic richness ranging from 4–16. Gene diversity ranged from 0.15 to 0.91. These primers provide powerful tools for examining the fine‐scale population structure and dynamics of Symbiodinium within a single host species.