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Isolation and characterization of 11 microsatellite loci from the black fly, Simulium negativum (Diptera: Simuliidae)
Author(s) -
SPIRONELLO MIKE,
RIZVI LEENA,
CURRIE DOUGLAS C.,
DAVY CHRISTINA M.,
MURPHY ROBERT W.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02544.x
Subject(s) - biology , microsatellite , black fly , simulium , locus (genetics) , loss of heterozygosity , genetics , allele , sibling species , polytene chromosome , zoology , evolutionary biology , chromosome , ecology , larva , gene
Eleven polymorphic microsatellite loci were isolated and developed from the black fly, Simulium negativum , a member of the Simulium arcticum sibling species complex. The observed heterozygosity of the 11 loci ranged from 0.03 to 0.83. The number of alleles per locus ranged from 8 to 19. Significant linkage disequilibria were encountered only for the primer pairs BF7‐1 with BF7‐5 and BF6‐32 with BF7‐16. Presumably, these microsatellite loci can be used to study genetic structure within the entire S. arcticum complex.