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Species‐specific primers for predation studies of the pollen beetle, Meligethes aeneus (Coleoptera, Nitidulidae)
Author(s) -
CASSELLUNDHAGEN ANNA,
ÖBERG SANDRA,
HÖGFELDT CAROL,
EKBOM BARBARA
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02540.x
Subject(s) - biology , pest analysis , predation , pollen , insect , mitochondrial dna , botany , zoology , biological pest control , ecology , gene , genetics
Species‐specific primers were developed for the pollen beetle (a pest in oilseed rape) for studies of predation by natural insect predators. Two forward and three reverse primers were designed within the mitochondrial COI gene and used in combination to amplify fragments in the size range of 163–290 bp. Remains of pollen beetle DNA were consistently detected in Pardosa spiders up to 24 h after ingestion but dropped drastically at 48 h. These primers will facilitate studies on biological control of this oilseed rape pest. Detection time was not correlated with fragment length as might be expected as the DNA gradually degrades into progressively shorter fragments over time.