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Isolation and characterization of polymorphic microsatellite loci in the freshwater fishes Telestes souffia and Telestes muticellus (Teleostei: Cyprinidae)
Author(s) -
DUBUT VINCENT,
MARTIN JEANFRANÇOIS,
COSTEDOAT CAROLINE,
CHAPPAZ RÉMI,
GILLES ANDRÉ
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02539.x
Subject(s) - biology , microsatellite , endangered species , cyprinidae , genetic diversity , evolutionary biology , zoology , genotyping , population , genetics , ecology , genotype , allele , fishery , habitat , gene , demography , fish <actinopterygii> , sociology
Ten novel polymorphic microsatellites (seven with perfect motifs) were isolated from vairone species ( Telestes souffia and Telestes muticellus ), which are endangered European cyprinid species. Together with 11 previously published cyprinid‐specific loci, five multiplex sets were optimized, allowing the genotyping of 21 polymorphic loci. The level of genetic diversity was assessed in 97 individuals from the two species T. souffia and T. muticellus . We also successfully applied the 21 microsatellites to nine related species. These primers will thus be useful in assessing population structure of the vairone and other cyprinid species, with application for conservation issues and phylogeographical approaches.