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Microsatellite loci within sixgill sharks, Hexanchus griseus
Author(s) -
LARSON SHAWN,
TINNEMORE DEBORAH,
AMEMIYA CHRIS
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02531.x
Subject(s) - squalus acanthias , biology , spiny dogfish , chondrichthyes , microsatellite , zoology , allele , genetics , gene , endocrinology
Novel sixgill shark ( Hexanchus griseus ) microsatellite loci were developed and tested on five shark species. A suite of microsatellite loci previously developed for lemon sharks ( Negaprion brevirostris ) was also tested. Data on 15 microsatellites are presented including primer sequences, number of alleles ( a ), observed ( H O ) and expected heterozygosities ( H E ), and F IS values for sixgill sharks ( a = 10–69, H O = 0.24–1.00, H E = 0.76–0.96 and F IS = –0.21–0.60), sevengill sharks ( Notorynchus cepedianus ) ( a = 6–40, H O = 0.20–0.73, H E = 0.59–0.94 and F IS = –0.47–0.58), Pacific spiny dogfish ( Squalus acanthias ) ( a = 3–13, H O = 0.00–0.96, H E = 0.24–0.93 and F IS =–0.52–1.00), angle sharks ( Squatina californica ) ( a = 1–4, H O = 0.00–1.00, H E = 0.60–1.00 and F IS =–1.00–0.25), and leopard sharks ( Triakis semifasciata ) ( a = 3–16, H O = 0.20–1.00, H E = 0.53–0.92 and F IS = –0.57–1.00). A final suite of 14 microsatellites (13 developed from sixgill sharks and one from lemon sharks) were found to be polymorphic and conform to Hardy–Weinberg equilibrium within sixgill sharks.