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Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas
Author(s) -
SAUVAGE C.,
BOUDRY P.,
LAPÈGUE S.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02525.x
Subject(s) - biology , crassostrea , pacific oyster , microsatellite , loss of heterozygosity , locus (genetics) , oyster , bay , genotyping , genetics , population , expressed sequence tag , population genetics , allele , zoology , evolutionary biology , fishery , genotype , gene , genome , civil engineering , demography , sociology , engineering
We report the development of 18 new polymorphic microsatellite DNA markers derived from Crassostrea gigas expressed sequences tags. Genotyping of 48 wild adult oysters sampled from Marennes‐Oléron bay (France) revealed 12 to 48 alleles per locus. Observed and expected heterozygosity levels ranged from 0.64 to 1 and from 0.77 to 0.97, respectively. The development of these new markers creates a useful complementary tool for population genetics studies, parentage analysis and mapping in Pacific oyster, a species of major aquacultural and ecological importance.