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Development of 52 new polymorphic microsatellite markers for the olive flounder, Paralichthys olivaceus
Author(s) -
KIM WOOJIN,
KANG JUNGHA,
NAM BOHYE,
KONG HEE JEONG,
PARK EUNMI,
LEE JEONGHO,
KIM KYUNGKIL,
KIM YOUNGOK,
HAN HYON SOB,
CHOI TAEJIN,
CHEONG JAEHUN
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02287.x
Subject(s) - paralichthys , biology , olive flounder , microsatellite , loss of heterozygosity , genetics , allele , population , genetic marker , gene , fish <actinopterygii> , fishery , demography , sociology
We characterized 52 new microsatellite markers isolated from (GT) n and (CT) n microsatellite‐enriched genomic libraries of the olive flounder ( Paralichthys olivaceus ). All markers were polymorphic, with eight to 30 (mean 15.1) alleles detected in 30 individuals from a single natural population. Observed heterozygosity ranged from 0.20 to 1.0. Segregation analysis within a mapping family revealed non‐amplifying null alleles at six loci. These results indicate that these new microsatellite markers will be useful for population genetic, parentage, and genome mapping studies.