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Variable microsatellite loci isolated from the Asian honeybee, Apis cerana (Hymenoptera; Apidae)
Author(s) -
TAKAHASHI JUNICHI,
SHIMIZU SOUTA,
KOYAMA SATOSHI,
KIMURA KIYOSHI,
SHIMIZU KIYOSHI,
YOSHIDA TADAHARU
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2009.02268.x
Subject(s) - biology , apis cerana , microsatellite , loss of heterozygosity , apidae , hymenoptera , allele , zoology , genetics , honey bees , botany , gene
We developed 12 polymorphic microsatellite loci for the Asian honeybee, Apis cerana using the magnetic particle method. Eight of these 12 were highly polymorphic, having four to seven alleles with an expected heterozygosity of 0.38 to 0.78. The primers also produce polymorphic products in related honeybee species such as Apis nigrocincta . These loci can be used to study parameters associated with genetic structure, such as paternity frequency and worker reproduction.