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Microsatellite loci for the white‐dotted mosquito ( Culex restuans ), a principal vector of West Nile virus in North America
Author(s) -
FONSECA DINA M.,
OKADA KENLI,
KRAMER LAURA D.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02512.x
Subject(s) - biology , microsatellite , flavivirus , vector (molecular biology) , culex , loss of heterozygosity , west nile virus , white (mutation) , arbovirus , genetics , primer (cosmetics) , virology , zoology , allele , virus , ecology , gene , larva , recombinant dna , chemistry , organic chemistry
We characterized the first microsatellite loci in the white‐dotted mosquito, Culex restuans , a critical early spring West Nile virus vector. An enrichment protocol yielded 960 positive clones of which we sequenced 300. We designed primers to amplify 29 unique di‐, tri‐ and tetranucleotide microsatellites and chose 17 that amplified consistently across populations and were polymorphic. We developed three multiplex primer combinations for all 17 loci. A survey of 44 individuals revealed two to 20 alleles across loci, and expected heterozygosity ranging from 0.17 to 0.89. These markers will allow examination of the life history of this mysterious early season encephalitis vector.