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Microsatellites from the charcoal rot fungus ( Macrophomina phaseolina )
Author(s) -
BAIRD RICHARD E.,
WADL PHILLIP A.,
WANG XINWANG,
JOHNSON DENITA H.,
RINEHART TIMOTHY A.,
ABBAS HAMED K.,
SHIER THOMAS,
TRIGIANO ROBERT N.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02511.x
Subject(s) - macrophomina phaseolina , biology , charcoal , fungus , microsatellite , botany , genetics , gene , allele , materials science , metallurgy
Microsatellite loci were identified from the charcoal rot fungus ( Macrophomina phaseolina ). Primer pairs for 46 loci were developed, and of these, 13 were optimized and screened using genomic DNA from 55 fungal isolates collected predominantly from two soybean fields in Mississippi. Twelve of the optimized loci were polymorphic and the number of alleles per locus ranged from 6 to 22. These microsatellites will be useful in population and pathogenicity studies to correspond with development of potential disease‐resistant soybean and other susceptible crops.