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Isolation and characterization of polymorphic microsatellite DNA markers in the sea squirt Halocynthia roretzi
Author(s) -
HAN HYONSOB,
NAM BOHYE,
KANG JUNGHA,
CHOI TAEJIN,
KIM WOOJIN,
HUR YOUNGBAEK,
YOON MOONGEUN
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02499.x
Subject(s) - biology , microsatellite , locus (genetics) , allele , population , genetics , isolation (microbiology) , zoology , evolutionary biology , gene , microbiology and biotechnology , demography , sociology
The sea squirt Halocynthia roretzi is an important marine food resource species that is found in the waters around Korea. We describe the isolation and characterization of 13 new polymorphic microsatellite loci in 96 sea squirt samples that were collected from the marine environment of Samcheok on the east coast of Korea. The number of alleles that were observed for each locus ranged from six to 32, and the value of expected and observed heterozygosities was 0.504–0.922 and 0.396–0.813, respectively. These markers will be useful tools for future population studies.