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Microsatellite loci for the stingless bee Melipona rufiventris (Hymenoptera: Apidae)
Author(s) -
LOPES DENILCE MENESES,
DA SILVA FILIPE OLIVEIRA,
FERNANDES SALOMÃO TÂNIA MARIA,
CAMPOS LÚCIO ANTÔNIO DE OLIVEIRA,
TAVARES MARA GARCIA
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02486.x
Subject(s) - stingless bee , biology , locus (genetics) , microsatellite , apidae , loss of heterozygosity , hymenoptera , population , zoology , allele , genetics , demography , gene , sociology
Eight microsatellite primers were developed from ISSR (intersimple sequence repeats) markers for the stingless bee Melipona rufiventris . These primers were tested in 20 M. rufiventris workers, representing a single population from Minas Gerais state. The number of alleles per locus ranged from 2 to 5 (mean = 2.63) and the observed and expected heterozygosity values ranged from 0.00 to 0.44 (mean = 0.20) and from 0.05 to 0.68 (mean = 0.31), respectively. Several loci were also polymorphic in M. quadrifasciata , M. bicolor , M. mandacaia and Partamona helleri and should prove useful in population studies of other stingless bees.