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Eight novel tetranucleotide and five cross‐species dinucleotide microsatellite loci for the ornate chorus frog ( Pseudacris ornata )
Author(s) -
DEGNER JACOB F.,
HETHER TYLER D.,
HOFFMAN ERIC A.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02478.x
Subject(s) - biology , microsatellite , locus (genetics) , allele , genetics , population , zoology , gene , sociology , demography
We describe the cloning and characterization of eight novel tetranucleotide microsatellite loci in the ornate chorus frog ( Pseudacris ornata ). We also screened 26 loci from GenBank that were isolated from other Pseudacris species and obtained consistent product from five of these dinucleotide loci. All loci are polymorphic. In our sample of 26 frogs from a natural population, polymorphism ranged from 1 to 22 alleles per locus with expected heterozygosities ranging from 0 to 0.958. These loci enable high‐resolution studies of P. ornata . Moreover, cross‐species amplification success suggests they will also be useful for other chorus frog species.