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Rapid polymerase chain reaction–restriction fragment length polymorphism method for discrimination of the two Atlantic cryptic deep‐sea species of scabbardfish
Author(s) -
STEFANNI SERGIO,
BETTENCOURT RAUL,
KNUTSEN HALVOR,
MENEZES GUI
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02470.x
Subject(s) - biology , restriction fragment length polymorphism , amplicon , restriction enzyme , polymerase chain reaction , terminal restriction fragment length polymorphism , genetics , microbiology and biotechnology , mitochondrial dna , restriction digest , restriction fragment , gene
The present investigation provides an efficient diagnostic method based on polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) analysis to discriminate between two cryptic species of scabbardfish, Aphanopus carbo and A. intermedius , with commercial relevance in several European fish markets. Two DNA fragments from the mtDNA, including control region and partial cytochrome oxidase subunit I genes of about 1100 bp and 700 bp, respectively, were isolated by PCR amplification. Digestion of the amplicon including the control region with Hae II and the amplicon including the COI gene with Sau 3AI restriction enzymes allowed an unequivocal discrimination between the two scabbardfish species. This PCR–RFLP method allowed a clear and rapid discrimination of the trichiurid species studied.