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Polymorphic microsatellite loci in the widespread amphidromous goby Sicyopterus lagocephalus and cross‐genus amplification among Sicydiinae
Author(s) -
HOAREAU T. B.,
BARBISAN F.,
DUBOIS S.,
ZANE L.,
BERREBI P.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02461.x
Subject(s) - biology , microsatellite , genotyping , goby , locus (genetics) , genus , allele , genetics , genomic dna , zoology , evolutionary biology , genotype , dna , gene , fishery , fish <actinopterygii>
Microsatellite DNA markers were isolated in an amphidromous goby ( Sicyopterus lagocephalus ) from a partial genomic library enriched for AC repeats. Eight microsatellites were highly polymorphic with six to 33 alleles per locus and expected heterozygosities ranging from 0.53 to 0.97. Cross‐species amplifications were performed within the sub‐family Sicydiinae by genotyping individuals from two species of the genus Cotylopus . Some of these loci were successfully amplified and showed polymorphism in the second genus.