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Isolation and characterization of microsatellite loci in Merluccius australis and cross‐species amplification
Author(s) -
MACHADOSCHIAFFINO G.,
GARCIAVAZQUEZ E.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02442.x
Subject(s) - biology , merluccius , microsatellite , hake , locus (genetics) , population , loss of heterozygosity , zoology , genetic structure , genetics , allele , genetic variation , fishery , gene , demography , sociology , fish <actinopterygii>
Eight novel and two heterologous microsatellite pairs of primers are presented for the Austral hake ( Merluccius australis ), representing the first microsatellite markers available for this species. Loci were characterized for 50 individuals from two populations in South America (Argentinean and Chilean coasts). All loci were polymorphic within M. australis (5 to 30 alleles per locus; observed heterozygosity between 0.320 and 0.840), and therefore useful for population genetic studies within the species. Cross‐species transferability was tested for 100 individuals from four additional species within the Merluccius genus ( M. albidus , M. bilinearis , M. gayi and M. hubbsi ), and results indicate that most of these primers pairs will likely be useful for population genetic studies on Merluccius species.