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Characterization of 32 novel microsatellite loci for population and mating system studies using Campostoma anomalum (central stoneroller)
Author(s) -
LANDIS J. B.,
HUDMAN S. P.,
GROSE M. J.,
SKALSKI G. T.,
WILEY E. O.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02430.x
Subject(s) - biology , microsatellite , locus (genetics) , loss of heterozygosity , mating system , evolutionary biology , allele , population , genetics , population genetics , mating , zoology , gene , demography , sociology
The central stoneroller ( Campostoma anomalum ) is an abundant, widespread and sexually dimorphic stream minnow that is a useful model for mating system studies as well as a sentinel species for understanding population‐level processes for fishes in headwater communities. We developed one genomic library enriched for dinucleotide repeats and isolated 48 putative, novel microsatellite loci. Of those, we present 32 polymorphic and independent microsatellite markers with 3 to 16 alleles per locus and heterozygosity ranging from 0.23 to 0.95. Hence, these markers will be useful for future behavioural, ecological and evolutionary studies using C . anomalum .