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Nineteen novel microsatellite markers for the Olympia oyster, Ostrea conchaphila/lurida
Author(s) -
STICK DAVID A.,
LANGDON CHRIS J.,
BANKS MICHAEL A.,
CAMARA MARK D.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02413.x
Subject(s) - biology , microsatellite , polymerase chain reaction , locus (genetics) , oyster , genetics , population , genetic marker , evolutionary biology , zoology , gene , fishery , allele , demography , sociology
Accurate evaluation of remnant Ostrea conchaphila/lurida population structure is critical for developing appropriate restoration efforts. Here we report 19 polymorphic microsatellites suitable for analyses of population differentiation, pedigree reconstruction and linkage map construction. We screened clones from four enriched genomic libraries, identified 73 microsatellite‐containing sequences and designed polymerase chain reaction primers for 44 of these loci. We successfully optimized polymerase chain reaction conditions for 20 loci, including one monomorphic locus. In a Willapa Bay reference sample, mean observed and expected heterozygosities were 0.6729 and 0.8377. Nine loci deviated from Hardy‐Weinberg equilibrium. These markers have proven useful for genetic studies of the Olympia oyster.