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Characterization of 35 novel microsatellite loci for ecological and evolutionary studies of the bluntnose minnow ( Pimephales notatus )
Author(s) -
LANDIS J. B.,
GROSE M. J.,
WILEY E. O.,
HUDMAN S. P.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02404.x
Subject(s) - biology , microsatellite , locus (genetics) , mating system , allele , evolutionary biology , minnow , ecology , zoology , genetics , mating , gene , fishery , fish <actinopterygii>
The bluntnose minnow ( Pimephales notatus ) is abundant and widespread with a broad ecological niche. This species is also sexually dimorphic with strong competition between males during the mating season. We developed two genomic libraries enriched for microsatellite repeats — one dinucleotide and one tetranucleotide — and isolated 48 putative, novel microsatellite loci. Of those, we present 35 polymorphic and statistically independent microsatellite markers with two to 18 alleles per locus and heterozygosities ranging from 0.04 to 1. These markers will be useful for future behavioural, ecological, evolutionary and mating system studies using P. notatus .