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Development of microsatellite markers in the Australasian snake‐necked turtle Chelodina rugusa and cross‐species amplification
Author(s) -
ALACS E. A,
HILLYER M. J.,
GEORGES A.,
FITZSIMMONS N. N.,
HUGHES J. M.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02389.x
Subject(s) - biology , microsatellite , locus (genetics) , linkage disequilibrium , gene flow , zoology , population , allele , genetics , genetic variation , gene , haplotype , demography , sociology
Seventeen microsatellite loci were developed for the snake‐necked turtle, Chelodina rugosa (Ogilby, 1890). Sixteen of the loci were polymorphic but three of these loci had null alleles. One locus displayed linkage disequilibrium. These 17 markers were tested for amplification in eight congeneric species with varying success; 98% amplification in Chelodina burrungandjii , 72% in C. canni , 38% in C. expansa , 58% in C. longicollis , 67% in C. mccordi , 73% in C. oblonga , 81% in C. parkeri , and 68% in C. pritchardi . These microsatellite markers will be useful for population assignment, gene flow, mating systems and hybridization studies in the genus Chelodina.