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Isolation and characterization of 13 polymorphic nuclear microsatellite primers for the widespread Indo‐Pacific three‐spot damselfish, Dascyllus trimaculatus , and closely related D. auripinnis
Author(s) -
LERAY MATTHIEU,
BELDADE RICARDO,
HOLBROOK SALLY J.,
SCHMITT RUSSELL J.,
PLANES SERGE,
BERNARDI GIACOMO
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02380.x
Subject(s) - damselfish , biology , microsatellite , pomacentridae , allele , coral reef , perciformes , zoology , genetics , evolutionary biology , coral reef fish , fish <actinopterygii> , ecology , fishery , gene
A set of 13 simple sequence repeat markers was developed from D. trimaculatus genomic DNA, tested for D. auripinnis and characterized using 40 individuals per species. All the loci were polymorphic with a number of alleles ranging from three to 30. Observed heterozygosities varied from 0.23 to 0.89 for D. trimaculatus and from 0.11 to 0.85 for D. auripinnis . Early results show that these will be powerful markers for the study of ecological and evolutionary mechanism in this coral reef fish species complex.