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Isolation and characterization of polymorphic microsatellite markers in the black spiny tailed iguana ( Ctenosaura pectinata ) and their cross‐utility in other Ctenosaura
Author(s) -
ZARZA EUGENIA,
PEREYRA RICARDO T.,
REYNOSO VICTOR H.,
EMERSON BRENT C.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02379.x
Subject(s) - biology , microsatellite , iguana , gene flow , locus (genetics) , loss of heterozygosity , population genetics , conservation genetics , population , genetic marker , zoology , genetic structure , allele , genetic variation , genetics , gene , demography , sociology
We isolated and characterized 10 polymorphic microsatellite loci from the Mexican black iguana ( Ctenosaura pectinata ) and assessed levels of polymorphism in sampling sites located in the northern areas of the species’ distribution range. Two to 19 alleles per locus and observed heterozygosity ranging from 0.15 to 0.96 were detected. These markers will be useful to describe population genetic structure, the extent of gene flow in contact zones, to study the mating system of the species and to address conservation genetics issues. Additionally, we evaluated the potential utility of these markers for studies of other species within the genus Ctenosaura (i.e. C. hemilopha, C. similis and C. oaxacana ).