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Characterization of 13 microsatellites for Lahontan cutthroat trout ( Oncorhynchus clarki henshawi ) and cross‐amplification in six other salmonids
Author(s) -
ROBINSON M. L.,
KIRCHOFF V. S.,
PEACOCK M. M.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02367.x
Subject(s) - biology , oncorhynchus , rainbow trout , subspecies , salmo , trout , microsatellite , threatened species , zoology , locus (genetics) , fishery , allele , ecology , genetics , fish <actinopterygii> , habitat , gene
Thirteen newly developed tri‐ and tetranucleotide repeat microsatellite markers were developed for Lahontan cutthroat trout ( Oncorhynchus clarki henshawi ), a threatened subspecies endemic to the Lahontan hydrographic basin in the western USA. These loci are highly polymorphic with five to 30 alleles per locus and observed heterozygosities ranging from 0.4 to 0.7. Cross‐species amplification of these markers was most successful in the closely related rainbow trout, Oncorhynchus mykiss , with only three loci amplifying in brown trout, Salmo trutta . Nonoverlapping allelic distributions for many of these loci among the six salmonid species screened suggest these markers may be useful for hybrid determination.