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Sixteen polymorphic microsatellite markers from Zizania latifolia Turcz. (Poaceae)
Author(s) -
QUAN ZHIWU,
PAN LEI,
KE WEIDONG,
LIU YIMAN,
DING YI
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1755-0998.2008.02357.x
Subject(s) - biology , microsatellite , amplified fragment length polymorphism , genetic diversity , gene flow , allele , poaceae , locus (genetics) , botany , population , genetic marker , genetics , genetic variation , gene , demography , sociology
Sixteen polymorphic microsatellite markers were isolated and identified in Zizania latifolia Turcz. (Poaceae), a perennial aquatic plant widespread in Eastern Asia. The microsatellite‐enriched library was constructed using the fast isolation by AFLP of sequences containing repeats method. These markers revealed two to 14 alleles, with an average of 5.6 alleles per locus. The observed and expected heterozygosities varied from 0.071 to 0.690 and from 0.174 to 0.812, respectively. These markers will be useful for studying of gene flow and evaluating the genetic diversity of the Zizania latifolia population.